Journal: Experimental Biology and Medicine

Article Title: Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice

doi: 10.3389/ebm.2024.10066

Figure Lengend Snippet: Study rabbit antibodies.

Article Snippet: CTSK (cathepsin K) , Novus Biologicals , NBP1-45460 , PAb , 1:100 , 10 mM sodium citrate, 1 h, 75°C.

Techniques:

Journal: BMC Oral Health

Article Title: MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement

doi: 10.1186/s12903-023-03443-8

Figure Lengend Snippet: Primer sequence

Article Snippet: Primary antibodies including anti-CTSK (1:500; LifeSpan BioSciences, USA), anti-NFATc1 (1:1000; Cell Signaling Technology, USA), and anti-SOCS1 (1:2000; Affinity Biosciences, USA) were used. β-actin (1:50000; Abclonal, China) was used as a control.

Techniques:

Journal: BMC Oral Health

Article Title: MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement

doi: 10.1186/s12903-023-03443-8

Figure Lengend Snippet: Compared with the BMMNCs without OC induction, the expression of miR-155 in BMMNCS after 3 days of induction showed an opposite trend with osteoclast-related markers. A The expression of osteoclast-related markers, including CTSK, TRAP, and NFATc1, was up-regulated in OC-treated cells, as detected by RT-PCR (p < 0.05), while miR-155 expression was significantly down-regulated (p < 0.05). B The RT-PCR results were visualized as a heat map to analyze the expression patterns, revealing the inverse trend of miR-155 compared to the other three genes. Student’s t-test, n = 3, ***P < 0.001, ****P < 0.0001

Article Snippet: Primary antibodies including anti-CTSK (1:500; LifeSpan BioSciences, USA), anti-NFATc1 (1:1000; Cell Signaling Technology, USA), and anti-SOCS1 (1:2000; Affinity Biosciences, USA) were used. β-actin (1:50000; Abclonal, China) was used as a control.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Oral Health

Article Title: MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement

doi: 10.1186/s12903-023-03443-8

Figure Lengend Snippet: After transfection with miR-155 mimic, the expression of osteoclast-related markers decreased. A The expression of osteoclast-related markers, including CTSK, TRAP, and NFATc1, was observed to decrease significantly in miR-155 mimic transfected cells (p < 0.05). B-C TRAP staining was performed on cells induced for osteoclast after 5 days of transfection, and a notable reduction in the number of TRAP-positive cells was observed. TRAP-positive cell area was quantified by Image J, the scale bars are 100 μm. D-E Western blotting showed that the expressions of osteoclast-related proteins CTSK and NFATc1 decreased after 5 days of osteoclast induction. Image J was used for densitometric analysis measurements. Student’s t-test, n = 3, *P < 0.05, **P < 0.01, ****P < 0.0001

Article Snippet: Primary antibodies including anti-CTSK (1:500; LifeSpan BioSciences, USA), anti-NFATc1 (1:1000; Cell Signaling Technology, USA), and anti-SOCS1 (1:2000; Affinity Biosciences, USA) were used. β-actin (1:50000; Abclonal, China) was used as a control.

Techniques: Transfection, Expressing, Staining, Western Blot

Journal: BMC Oral Health

Article Title: MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement

doi: 10.1186/s12903-023-03443-8

Figure Lengend Snippet: After transfection with miR-155 inhibitor, the expression of osteoclast-related indicators increased. A The expression of the osteoclast-related marker CTSK was observed to increase, while miR-155 was decreased in cells transfected with the inhibitor reagent for 48 h followed by osteoclast induction for 3 days (p < 0.05). However, there was no significant difference in the expression of TRAP and NFATc1. B-C TRAP staining was performed on cells induced for osteoclast for 5 days after transfection, and a notable increase in the number of TRAP-positive cells was observed. TRAP-positive cell area was quantified by Image J, the scale bars are 100 μm. D-E Western blotting showed that the expressions of osteoclast-related proteins CTSK and NFATc1 were increased after 5 days of osteoclast induction. Image J was used for densitometric analysis measurements. Student’s t-test, n = 3, *P < 0.05, ****P < 0.0001

Article Snippet: Primary antibodies including anti-CTSK (1:500; LifeSpan BioSciences, USA), anti-NFATc1 (1:1000; Cell Signaling Technology, USA), and anti-SOCS1 (1:2000; Affinity Biosciences, USA) were used. β-actin (1:50000; Abclonal, China) was used as a control.

Techniques: Transfection, Expressing, Marker, Staining, Western Blot

Journal: BMC Oral Health

Article Title: MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement

doi: 10.1186/s12903-023-03443-8

Figure Lengend Snippet: AntagomiR-155 increased osteoclast-related markers in gingival tissues. A The expression of miR-155, as well as osteoclast-related markers CTSK and TRAP, was found to be increased in the AntagomiR-155 group (TM 5d + AntagomiR-155) compared to the orthodontic group (TM 5d). B-C Western blotting analysis revealed that TM 5d + AntagomiR-155 resulted in an upregulation of the osteoclast-related protein NFATc1 in gingival tissues. D At the other time point, the AntagomiR-155 group (TM 12d + AntagomiR-155) showed significantly decreased miR-155 expression and increased osteoclast-related marker NFATc1 expression compared with the orthodontic group (TM 12d). There was also a tendency for increased TRAP expression. E-F Western blotting showed that TM 12d + AntagomiR-155 increased the expression of osteoclast-related protein CTSK in gingival tissues. Student’s t-test, n = 3, *P < 0.05, **P < 0.01, ****P < 0.0001

Article Snippet: Primary antibodies including anti-CTSK (1:500; LifeSpan BioSciences, USA), anti-NFATc1 (1:1000; Cell Signaling Technology, USA), and anti-SOCS1 (1:2000; Affinity Biosciences, USA) were used. β-actin (1:50000; Abclonal, China) was used as a control.

Techniques: Expressing, Western Blot, Marker